Praveena Ch, Swaroopa Rani S and Veeresham C
Calophyllum inophyllum Linn. is an economically and medicinally important species of genus Calophyllum, family Guttiferae. The plant is known for its abundant traditional medicinal value, as well as for its pharmacological activities, such as antiviral, cytotoxic, anti-platelet aggregation, etc. Phytochemical literature investigation of the plant revealed the presence of various compounds, such as coumarins, xanthones, triterpenes and steroids. However, steroidal glycosides are not reported from this plant. In search of new chemical compounds, phytochemical investigation of methanolic extract of leaves was carried out. After repeated column chromatography using Hexane and Hexane:Ethyl acetate mixtures of different proportions as eluents, resulted in isolation of three compounds suspected to be steroidal compounds. The homogeneity of isolated compounds was established by TLC and HPLC, as there was no interference of spot or peak in chromatograms. For detailed structure elucidation, UV, IR, one dimensional H1NMR, C13 NMR and MS spectras were recorded for structure elucidation.