Islam Minarul , Manirujjaman M , Khan MMH , Rahman Matiar, Amin M Ziaul , Uddin AMM , Biswas Shahangir and Islam MA
Diabetes mellitus is a multifarious disease. There is no specific cause for the development of this disease. Fish oil plays important role for the remediation of diabetes mellitus. Conjugated Linoleic Acid (CLA) was synthesized by five steps that included saponification of soybean oil, low temperature crystallization of saturated fatty acid, urea crystallization of oleic acid, alkali isomerization of linoleic acid to CLA and fatty acid analysis. Fish oil was extracted from sun dried fish flesh by n-hexane. The rats were divided into five groups. The Group-1 and Group-2 considered as normal and Diabetic Control. Group-3, Group-4 and Group-5 diabetic rats were treated with CLA, Fish oil and Glibenclamide respectively. Diabetes Mellitus (DM) was induced by single intra peritoneal injection of freshly prepared alloxan hydrate (55 mg/kg Body Weight of rat) in 0.9% saline solution. The significant effects (P<0.001) of CLA and fish oil were observed in alloxan-induced diabetic rats to control the blood glucose level within range. Antilipidemic effects of CLA and fish oil were also observed and found to reduce the cholesterol and LDL level whereas increased the HDL level significantly (P<0.05). Like glibenclamide CLA and fish oil had significant effects on the control of alloxan induced diabetes mellitus in Sprague Dawley rats.